Journal: iScience

Article Title: A central role for Numb/Nbl in multiple Shh-mediated axon repulsion processes

doi: 10.1016/j.isci.2025.112293

Figure Lengend Snippet:

Article Snippet: Mouse anti-Shh , Developmental Studies Hybridoma Bank , Cat# 5E1 RRID: AB_528466.

Techniques: Virus, Recombinant, Molecular Weight, Concentration Assay, Mutagenesis, shRNA, Software, Electroporation

Journal: Journal of Clinical Medicine

Article Title: Reduced Salivary Gustin and Statherin in Long-COVID Cohort with Impaired Bitter Taste

doi: 10.3390/jcm13226816

Figure Lengend Snippet: ( A ) Epithelial cells in saliva stained with AO/PI. Majority of the epithelial cells are viable as indicated by AO+ staining. ( B ) A representative image of cell in saliva staining positive for SHH and ( C ) occludin.

Article Snippet: Immunofluorescence was performed using primary antibodies against pan cytokeratin (1:100; pan-Cytokeratin Antibody (AE1/AE3): sc-81714, Santa Cruz Biotechnology, Inc.; Dallas, TX, USA), SHH (Catalog #: AF464, R&D Systems, Minneapolis, MN, USA), and occludin (1:250: sc-133256, Santa Cruz Biotechnology, Inc.; Dallas, TX, USA).

Techniques: Staining

Journal: Cell reports

Article Title: Alterations of ceramide synthesis induce PD-L1 internalization and signaling to regulate tumor metastasis and immunotherapy response

doi: 10.1016/j.celrep.2024.114532

Figure Lengend Snippet: (A) Shh protein abundance in SCR or CerS4 shRNA-transfected 4T1 cells was detected in western blots. Data quantification is shown in right panel ( n = 3). (B) ELISA detected shh protein in the cell-culture supernatant from SCR or CerS4 shRNA-transfected 4T1 cells ( n = 4). (C) Representative images of migration assay measured in fibronectin-coated Boyden chambers ( n = 3). Scale bars, 10 μm. (D) Interaction of Shh and PTCH1 was detected by a co-immunoprecipitation with a Shh-targeting antibody and blotting for Shh and PTCH1 in 4T1 cells stably transfected with SCR or CerS4 shRNA ( n = 2). Data quantification is shown in right panel. (E) Effect of exogenous shh ligand (100–200 μg/mL) on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA ( n = 3). (F) Effect of a neutralizing SMO and pSMO targeting antibody on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA. Data quantification isshown in right panel ( n = 3). Scale bars, 10 μm. (G) Effect of exogenous shh ligand (200 μg/mL) on cellular migration in 4T1 cells stably expressing SCR or CerS4 shRNA with or without neutralizing pSMO antibody treatment ( n = 2). (H) TGFBR1 protein abundance in SCR or CerS4 shRNA-transfected 4T1 cells was detected by western blot. Data quantification is shown in lower panel ( n = 4). (I) Interaction of pSmo and TGFBR1 proteins was measured using a PLA in 4T1 cells stably expressing SCR or CerS4 shRNA with or without neutralizing pSMO antibody treatment. Data quantification is shown in right panel ( n = 3). Scale bars, 5 μm. (J) Cell-surface expression of SMO and TGFBR1 were determined using flow cytometry in 4T1 cells stably expressing SCR or CerS4 shRNA ( n = 3). Data represent means ± SD; Student’s t test or two-way ANOVA was used to determine significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant.

Article Snippet: Mouse Sonic Hedgehog/Shh N Terminus Quantikine ELISA Kit , Novus Biologicals , Cat# MSHH00.

Techniques: Quantitative Proteomics, shRNA, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Migration, Immunoprecipitation, Stable Transfection, Expressing, Flow Cytometry

Journal: Cell reports

Article Title: Alterations of ceramide synthesis induce PD-L1 internalization and signaling to regulate tumor metastasis and immunotherapy response

doi: 10.1016/j.celrep.2024.114532

Figure Lengend Snippet:

Article Snippet: Mouse Sonic Hedgehog/Shh N Terminus Quantikine ELISA Kit , Novus Biologicals , Cat# MSHH00.

Techniques: Recombinant, Red Blood Cell Lysis, Lysis, Clinical Proteomics, Staining, Transfection, In Situ, Proximity Ligation Assay, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, RNA Sequencing, Sequencing, shRNA, Software